RNA-Seq Data Analysis in R (2-days online workshop)

The course is recommended for researchers who are familiar with programming in R or have completed our introductory course in R. The workshop will start with a presentation on the specificity of high-throughput data analysis such as RNA-Seq data. We will introduce you to the comprehensive analysis of the transcriptome such as quality control, mapping, initial filtration and normalization or higher-order analysis, i.e. differential analysis and gene ontology. We teach practical knowledge supported by examples, exercises and the so-called case study. The goal of the course is to introduce the essentials of next generation sequencing (NGS) data analysis. The classes are in the form of a workshop, with participants’ progress checking and the possibility of asking questions. After the end of the workshop, participants will receive a certificate confirming their attendance in the course.

Date: to be agreed with the client (2-days online workshop).

Time: 10 hours (5 hours each day with a coffee break).

Location: online.

Group: for a group larger than 3 persons. .

Teacher: Arkadiusz Kajdasz, Ph.D.

Price: 380 EUR/USD (plus VAT if applicable).


  • Introduction to RNA-Seq and Bioconductor
  • Analysis of sequencing quality
  • Mapping and reads counting
  • Pre-processing of counts
  • Essential statistical tests for NGS data: edgeR, DEseq, limma
  • GO and KEGG analysis
  • Results visualization
  • Case study

Arkadiusz Kajdasz, Ph.D.

Works in the Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Poznań. He gained teaching experience working with students at the Adam Mickiewicz University, Poznań. Interested in the metabolism and post-transcriptional modifications of RNA. Practitioner in the analysis of RNA-Seq data. He participated in many bioinformatics courses organized in Poland and abroad (e.g., in Ontario Institute for Cancer Research, Toronto, Canada). He collaborates with the Poznań University of Life Sciences and the Military Institute of Medicine in Warsaw.